drug-resistant hiv-1 rt gene mutations in patients under treatment with antiretroviral drugs (haart) in iran

نویسندگان

رضا گل محمدی

gol mohammadi, r school of medicine, golestan university of medical sciences, gorgan, iranدانشکدۀ پزشکی علیجان تبرائی

tabaraei, a infectious diseases research centerمرکز تحقیقات بیماریهای عفونی عبدالله عباسی

abbasi, a infectious diseases research centerمرکز تحقیقات بیمارهای عفونی ناهید خادمی

khademi, n kermanshah, iranمدیر گروه بیمارها ی استان کرمانشاه بهزاد مهدویان

چکیده

abstract background and objective: highly active antiretroviral therapy (haart) can effectively prevent the progression of hiv-1 replication and increase life expectancy. there are numerous causes of treatment failure and the leading one is drug resistance. thus, we aimed to determine the hiv rt gene drug resistance mutations in patients treated with antiretroviral medications. material and methods: in this cross - sectional study, venous blood was taken from 130 hiv-positive patients treated with antiretroviral medications. in order to determine drug resistance mutations, rt-pcr and pcr steps were performed using rt gene specific primers. subtypes and mutations in the virus genome were determined using the stanford hiv drug resistance sequence database. results: in 122 treating patients, most of the major mutations were associated with nucleoside and non-nucleoside drugs. subtype a in 66.4%, subtype d in 26.2% and subtype b in 7.4% of the participants were reported. they were resistant to nucleoside rt inhibitor drugs (23.7%) and non-nucleoside rt inhibitor drugs(30.3%). the highest were related to nevirapine (21.3%) and efavirenz (19.7%) and the lowest to both tenofovir and zidovudine (91.5%). conclusion: the use of two nucleoside rt inhibitor drugs combined with one protease inhibitor drug could be effective in the treatment of haart. key words: hiv, nucleoside rt inhibitor, non- nucleoside rt inhibitor

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Drug-Resistant HIV-1 RT Gene Mutations in Patients under Treatment with Antiretroviral Drugs (HAART) in Iran

Abstract Background and Objective: Highly Active Antiretroviral Therapy (HAART) can effectively prevent the progression of HIV-1 replication and increase life expectancy. There are numerous causes of treatment failure and the leading one is drug resistance. Thus, we aimed to determine the HIV RT gene drug resistance mutations in patients treated with antiretroviral medications. Material...

متن کامل

Drug Resistance Profile and Subtyping of HIV-1 RT Gene in Iranian Patients under Treatment

Identification of drug resistant mutations is important in the management of HIV-1 infected patients. The aim of the current study was to evaluate drug resistance profile of RT gene and assess subtype among HIV-1 circulating strains and intensification of physician’s options for the best therapy. HIV-1 RNA of 25 sampleswas extracted from plasma and RT Nested- PCR was performed and the fin...

متن کامل

Drug- Resistance- Associated Mutations and HIV Sub-Type Determination in Drug-Naïve and HIV-Positive Patients under Treatment with Antiretroviral Drugs

Abstract Background and Objective: Resistance to antiretroviral agents is a significant concern in clinical management of HIV-infected individuals. Resistance is the result of mutations that develops in the viral protein targeted by antiretroviral agents. Material and Methods: In this cross-sectional study, the blood samples of 40 HIV-positive patients were collected. Twenty of them were d...

متن کامل

Antiretroviral Drug Resistance Mutations among HIV Treatment Failure Patients in Tehran, Iran

BACKGROUND This study aimed to determine drug resistance mutations in patients with virological failure and find correlation between HIV drug resistance test and viral load. METHODS Blood sample was collected from 51 patients who suspicious treatment failure in the center of Imam Khomeini Hospital, Tehran, Iran in 2015. Viral voluntary counseling and testing load test was done and the patient...

متن کامل

Viraemia and HIV-1 drug resistance mutations among patients receiving antiretroviral treatment in Mozambique.

This study was conducted among individuals taking first-line antiretroviral treatment (ART) for at least 12 months under programme conditions in Maputo, Mozambique in order to report on the level of detectable viraemia and the proportion and types of drug resistance mutations among those with detectable viral loads. HIV-1 RNA viral load levels (lower detection limit <50 copies/ml) were measured...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
medical laboratory journal

جلد ۹، شماره ۱، صفحات ۱-۸

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023